The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cellcell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic Tcell development, and both tumor progression and suppression.
Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Deltalike protein1(DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAMfamily metalloproteases and the gammasecretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus.
Subsequent signal transduction then occurs through either the CSLNICDMastermind complex cascade (canonipathway),NFkappaBNICD and CSLNICDDeltex complex signaling cascades (noncanonical pathway).
The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the noncanonical pathway promotes differentiation.DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in celltocell communication in mammalian embryos.
DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes.
Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 1x10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Hybridoma:P3U1 myeloma cells with spleen cells from Armenian hamsters.
Storage and Stability:Store product at 4℃ before and after reconstitution.
DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4℃.
Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：hamster
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from tissue culture supernatant.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1mL sterile ddH2O.
Specificity：Recognizes mouse Delta-like protein1 (DLL1). Species Crossreactivity: rat. Weakly recognizes human DLL1.
