Desmoplakin (UniProt: P15924; also known as DP, 250/210 kDa paraneoplastic pemphigus antigen) is encoded by the DSP gene (Gene ID:1832) in human.
Desmoplakin is a major high molecular weight homodimeric protein that is involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes, which are prominent adhesive junctions present between many epithelial cells and cardiomyocytes.
Desmoplakin is the most abundant of the desmosomal proteins and plays a key role in tissue morphogenesis and maintaining the integrity of tissue.
Desmoplakin is composed of an N-terminal plakin domain, a central alpha-helical coiled-coil rod domain, and a C-terminal intermediate filament-binding domain.
The N-terminal domain is required for desmosome association and the C-terminal binds to desmin.
Three isoforms for Desmoplakin have been reported that are produced by alternative splicing.
Desmoplakin 2 and 3 lack most of the central rod domain.
Desmoplakin 1 is the dominant isoform in the cardiac tissue.
Desmoplakin is phosphorylated by protein kinase A on serine 2849 and this phosphorylation affects its association with epidermal, simple cytokeratins, and VIM intermediate filaments.
Mutations in DSP gene have been linked to DCWHK (Cardiomyopathy, dilated, with woolly hair and keratoderma) that is characterized by a generalized striate keratoderma particularly affecting the palmoplantar epidermis, woolly hair, and dilated left ventricular cardiomyopathy.
Mice deficient in Desmoplakin perish around the time of implantation (E6.5) and show fewer desmosomes.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunocytochemistry: 1:500 detected Desmoplakin in A431 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Desmoplakin, targets an epitope within the C-terminal half.
Clone # USB：20B6
Isotype：IgG1,k
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：His-tagged recombinant protein fragment corresponding to 863aa from the C-terminal half of human Desmoplakin.
