Toll like receptors are a family of pattern-recognition receptors involved in the activation and regulation of inflammatory and immune responses activated by invasion of host tissues by microbial pathogens.
TLR2 plays a role in a range of infections including gram-positive bacteria, mycobacteria, and yeast.
It is activated by PAMPs, signals to NF-kappa-B which in turn induces cytokine secretion and the inflammatory response.
This clone was used to characterize the expression of porcine TLR2; cells of the innate immune system - monocytes, macrophages, and granulocytes, but not on peripheral blood lymphocytes were found to express porcine TLR2.
Swine neutrophils express low levels of TLR2.
It is also expressed on epithelial cells lining the tracheobronchial and intestinal tracts, bile ducts in the liver and renal tubules, and on the basal layer of the epidermis.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from tissue culture supernatant.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes porcine CD282.
Clone # USB：1H11
Isotype：IgG1
Calc Applications Abbrev：FC
Calc Crossreactivity：Po
Immunogen：NIH/3T3 mouse cells transfected with plasmid pTLR2-GFP, encoding the porcine TLR2 tagged with the green fluorescent protein (GFP) on the carboxy-terminal end
