Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution.
The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation.
052323 displays high affinity and binds the PEG backbone and can be employed as a detection antibody for the quantification of PEG.
It binds to a smaller epitope of the PEG backbone when compared to other antibodies.
Since it binds to a smaller PEG epitope (~750 Da), it is not compatible with detergents that contain an ethylene oxide backbone, such as Tween 20.
Instead 0.05% CHAPS is a more suitable detergent when using this antibody.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 5ug/ml of Polyethylene Glycol (PEG) (10K-PEG, 3K-PEG, and 2K-mPEG) were detectedOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.04% sodium azide.
Specificity：Recognizes proteins conjugated with polyethylene glycol (PEG).
Clone # USB：6.3
Isotype：IgG1,k
Calc Applications Abbrev：E
Immunogen：PEG-conjugated to E.coli beta-glucuronidase.
