Norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks.
Murine norovirus (MNV-1) that is about 28 to 35 nm in diameter, shares many biochemical and genetic features with human noroviruses.
An analysis of the MNV-1 genome shows three open reading frames (ORF) that are characteristic of noroviruses and vesiviruses.
ORF1 is reported to encode a predicted 187kD polyprotein that contains the 2C helicase, 3C protease, and 3D polymerase motifs.
ORF2 encodes a 59kD capsid protein that can self-assemble into virus-like particles when expressed in a baculovirus expression system.
ORF3 is reported to encode a putative 22kD basic protein.
The norovirus capsid protein contains a highly conserved shell (S) and protruding (P) domain and the P domain is shown to contain binding sites for receptors and neutralizing antibodies.
The S domain is shown to be highly conserved and the capsid sequence diversity is limited to the P domain.
MNV-1 infection is reported to modulate the MAPK pathway to activate eIF4E phosphorylation and the activation of p38 and Mnk during MNV-1 infection is considered to be important for its replication.
Applications:Suitable for use in Neutralization, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Highly Purified
Purity：Purified by size-exclusion chromatography
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes the P domain portion of the viral capsid protein and effectively neutralize MNV-1 and WU20 strains. It can also neutralize recombinant viruses V339I and D348E at higher doses.
