83-5983-15　Norovirus (MNV-1) 100ug　052319

特徴

• Norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks.
• Murine norovirus (MNV-1) that is about 28 to 35 nm in diameter, shares many biochemical and genetic features with human noroviruses.
• An analysis of the MNV-1 genome shows three open reading frames (ORF) that are characteristic of noroviruses and vesiviruses.
• ORF1 is reported to encode a predicted 187kD polyprotein that contains the 2C helicase, 3C protease, and 3D polymerase motifs.
• ORF2 encodes a 59kD capsid protein that can self-assemble into virus-like particles when expressed in a baculovirus expression system.
• ORF3 is reported to encode a putative 22kD basic protein.
• The norovirus capsid protein contains a highly conserved shell (S) and protruding (P) domain and the P domain is shown to contain binding sites for receptors and neutralizing antibodies.
• The S domain is shown to be highly conserved and the capsid sequence diversity is limited to the P domain.
• MNV-1 infection is reported to modulate the MAPK pathway to activate eIF4E phosphorylation and the activation of p38 and Mnk during MNV-1 infection is considered to be important for its replication.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/mL detected Norovirus (MNV-1) in RAW 264.7 cells infected with Murine norovirus-1.CW3
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.