Recombinant monoclonal antibody to Norovirus capsid protein VP1.
Manufactured with variable regions (i.e.
specificity) from human B cell clone A1227.
Norovirus (NoVs) is one of the main pathogens causing sporadic cases and large outbreaks of acute gastroenteritis, and norovirus can infect people of all ages.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Application Notes:ELISA was used to determine the binding specificity of the antibody for variety of GI and GII genotype VLP.
This antibody is capable of recognizing GI.1, GI.3, GI.4, GII.1, GII.2, GII.3, GII.4c, GII.14, GII.17 in an ELISA.
This antibody is not capable of neutralizing Norovirus (PMID: 31216462).
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% Proclin 300.
Specificity：Recognizes Norovirus capsid protein VP1. Shows broadly binding but no blocking activity toward multiple GI and GII strains such as GI.I, GII.2, and GII.17. This antibody recognizes a highly conserved epitope located at an occluded site on the norovirus capsid, at the interface between the P domain and shell domain of the Norovirus, which explain its high level of cross-reactivity.
Clone # USB：A1227 (recomb)
Isotype：IgG1,k
Calc Applications Abbrev：E
Immunogen：Isolated from an anti-NoV GII.4c IgG serum antibody repertoire generated from three donors that experienced a significant increase in GII.4 titer after immunization with the bivalent GII.4c + GI.1 VLP vaccine.
