83-5983-13　APC (Adenomatous Polyposis coli) 100ug　052316

特徴

• The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon.
• These polyps invariably progress to colon cancer in addition to other extracolonic manifestations.
• The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS.
• APC has been found to be associated with structural components of intracellular junctions.
• Functions as a tumor suppressor, promoting rapid degradation of CTNNB1 and is a negative regulator of Wnt signaling.
• Also plays a role in HGF-induced cell migration.
• Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells.
• Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
• It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
• Applications:Suitable for use in Immunofluorescence, Western Blot and Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 1-2ug/10e6 cells in 100ulImmunofluorescence: 1-2ug/mlWestern Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.