S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates.
The protein exists in both heterodimeric and homodimeric forms.
The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells.
The beta subunit is present in all S-100 positive cells and tumors.
In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain.
In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain.
The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
Applications:Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:100-1:200 Western Blot: 1:1000 detected S100 on 10ug of PC12 lysate
Optimal dilution determined by the researcher.
Positive Control:Melanoma
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide with antibody stabilizer.
Specificity：Reccognizes ~22kD (non-reduced) or 11kD (reduced) of alpha and beta bovine S100. Species Crossreactivity: human, rat and mouse
