83-5983-11　Double-stranded RNA (dsRNA) 100ul　052313

特徴

• Double-stranded RNA (dsRNA) is a viral product that induces innate immunity, leading to the production of interferon (IFN) alpha and beta, which can lead to the activation of hundreds of IFN-stimulated genes that confer resistance to viruses.
• dsRNA of more than 30-bp length are reported to be is a key activator of the innate immune response against viral infections.
• dsRNA is produced by positive-strand RNA viruses, dsRNA viruses, and DNA viruses.
• However, negative-strand RNA viruses are not shown to generate any significant dsRNA signals.
• The interaction of the host cell with dsRNA can occur in several ways.
• Mainly, specific receptors activate the synthesis of IFN alpha and beta, antiviral proteins, and dsRNA-activated enzymes that can block viral replication.
• dsRNA replication is shown to occur in the cytoplasm for all dsRNA viruses.
• This dsRNA-specific mouse monoclonal antibody specifically recognizes dsRNA of more than 40-bp length.
• 052313 has been used to detect dsRNA intermediates of multiple types of viruses, including Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, and Polio virus in biological samples.
• It can be useful in understanding how anti-viral responses are initiated and what how viruses overcome and avoid these antiviral therapies.
• Applications:Suitable for use in Immunocytochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry/Immunofluorescence: 1:60 detected dsRNA in Dengue virus infected A549 cells
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.