Merkel cells are neuroendocrine cells found in skin that have synaptic contacts with somatosensory afferents.
These cells can turn malignant and form an aggressive form of skin cancer, which is known as Merkel cell carcinoma (MCC).
A vast majority of MCC are caused by a polyomavirus known as Merkel cell polyomavirus (MCPyV or MCV).
The MCPyV genome is reported to undergo clonal integration into the host cell chromosomes of MCC tumors and expresses small T antigen and truncated large T antigen.
Full-length MCPyV large T-antigen is a 125kD nuclear protein, however, MCPyV T-antigens obtained from MCC have natural truncating mutations, which result in variably-sized, smaller proteins.
Based on homology to other polyomaviruses, the MCPyV large and small T antigens are predicted to be oncogenic and contribute directly to the carcinogenesis of MCC.
MCPyV large T antigen can serve as a specific marker for MCC.
Applications:Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml detected MCPyV large T-antigen in 10ug of MKL-1 cell lysates
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes MCPyV Large T-Antigen, highly specific for MCPyV large T and 57kT isoforms, but does not detect MCPyV small T antigen..
Clone # USB：CM2B4
Isotype：IgG2b,k
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated linear peptide corresponding to RSRKPSSNASRGA sequence from the Merkel cell polyomavirus large T antigen exon 2 with a C-terminal cystiene.
