83-5982-80　IL17A (Interleukin-17A) (PE) 100Tests　052268-PE

特徴

• Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells.
• IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins.
• It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi.
• It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites.
• High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19.
• This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells.
• It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 10ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspension.
• Intracellular staining
• Optimal dilutions to be determined by the researcher.
• Excitation Laser:Blue (488nm)
• Storage and Stability:Store product at 4℃ in the dark.
• DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.