Tested using A375 cellsImmunohistochemistry: 8-25ug/ml for immersion fixed paraffin-embedded sections of human skin cancer tissue (melanoma) Western Blot: 1ug/ml in A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form:Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.