CD44 antigen (UniProt: P16070; also known as CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, CD44) is encoded by the CD44 (also known as LHR, MDU2, MDU3, MIC4) gene (Gene ID: 960) in human.
CD44 is a single-pass type I, highly glycosylated membrane protein that is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa21-649), a transmembrane domain (aa650-670), and a cytoplasmic domain (aa671-742).
Nineteen different isoforms of CD44 have been described that are produced by alternative splicing.
All isoforms possess a hyaluronic acid (HA)-binding domain in its N-terminal region in the extracellular domain.
CD44 expression is observed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells.
It binds to several ligands including hyaluronic acid (HA), Osteopontin, chondroitin, collagen, fibronectin, and serglycin/sulfated proteoglycan.
However, the main ligand for CD44 is HA that is expressed by stromal and cancer cells.
HA binds the CD44 ligand binding domain and induces conformational changes that allow binding of adaptor proteins or cytoskeletal elements to intracellular domains.
This leads to the activation of various signaling pathways leading to cell proliferation, adhesion, migration, and invasion.
Overexpression of CD44 has been reported in several cell types and it is considered as an ideal marker for cancer stem cells.
Its interaction with appropriate extracellular matrix ligands promotes the migration and invasion processes involved in tumor metastases.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Inhibition, Flow Cytometry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500 detected CD44 in human fetal foreskin tissue lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes human CD44. Species Crossreactivity: mouse
Clone # USB：HERMES-3
Isotype：IgG2a,k
Calc Applications Abbrev：E FC IC IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Purified Hermes-1 antigen from KCA, a human B lymphoblastoid line. Epitope: Extracellular domain
