Pro-HGF-A is a 96kD member of the PA-FXII-HGFA family of enzymes.
It is secreted by hepatocytes and is found in plasma at ~40nM concentration.
Other cells selectively reported to express HGF-A include astrocytes, endothelium, fibroblasts and macrophages.
Upon activation, HGF-A is known to activate both proHGF and proMSP via proteolytic cleavage.
The circulating 96kD form of mouse HGF-A represents an inactive 619aa proform aa35-653.
Within this proform exists one FN type II domain aa100-147, an EGF-like domain aa157-195, an FN type III domain aa197-237, a second EGF-like domain aa238-276, a kringle domain aa283-364 and a functional peptidase S1 domain aa406-644.
Activation of HGF-A typically occurs with a thrombin-mediated cleavage between aa405-406 and a KLK cleavage between aa369-370.
This creates a 34kD heterodimeric active product that contains a 2kD N-terminus aa370-405 disulfide linked to a 32kD C-terminus aa406-653.
Once activated, the 34kD heterodimer binds to cell surface HAI-1, rendering it unavailable to circulating HGF.
When needed, this HGF-A:HAI-1 complex is released, HGF-A dissociates from HAI-1, and HGF-A is free to activate proHGF.
Over aa35-653, mouse HGF-A shares 92% and 82% aa identity with rat and human HGF-A.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1mL sterile PBS.
Specificity：Recognizes mouse Pro-HGF Activator. Only the HGFA precursor is recognized. Does not react with the active form of recombinant mouse HGFA, recombinant human HGFA, or recombinant human Kallikreins 3, 5, and 11.
Clone # USB：24L1
Isotype：IgG2a
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln35-Ser653 (predicted) from mouse Pro-HGF Activator, expressed in S. frugiperda insect ovarian cell line Sf21.
