Arrestin-C (UniProt: A0A2K5U9A7; also known as Cone arrestin, Retinal cone arrestin-3) is encoded by the ARR3 gene (Gene ID: 102134564) in Monkey.
Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G-protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins.
Arrestin-C is predominantly found in inner and outer segments, and the inner plexiform regions of the retina.
It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision.
Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes.
Several structural elements are reported to contribute to arrestin binding.
The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor.
The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region.
Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity.
Clone 7G6 is a cone-specific marker that recognizes all cones in the adult primate retina.
In fetal retina, although long and middle wavelength-sensitive cones recognized, a subset of short wavelength-sensitive cones are shown to be delayed in their reactivity to this clone.
Applications:Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:500 detected Arrestin-C in frozen monkey retina tissuesOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：monkey
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes cone arrestin (Arrestin-C). Targets an epitope within the N-terminal region. Species Crossreactivity: Rhesus macaque, human and bovine
Clone # USB：7G6
Isotype：IgG1,k
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Bo Hu Mk
Immunogen：Crude retinal extract from Monkey (Macaca fascicularis). Epitope: N-terminal
