Free fatty acid receptor 3 (UniProt O14843; also known as G-protein coupled receptor 41) is encoded by the FFAR3 (also known as GPR41) gene (Gene ID 2865) in human.
Free fatty acids (FFAs) act as both energy sources and signaling molecules via G-protein-coupled receptors (GPCRs) called FFARs (FFA receptors).
FFAs are categorized by the length of their carbon chains into short-chain FAs (SCFAs; less than 6 carbons), medium-chain FAs (MCFAs; 6–12 carbons), and long-chain FAs (LCFAs; more than 12 carbons).
MCFAs and LCFAs activate FFAR1 and FFAR4, whereas SCFAs activate FFAR3 and FFAR2.
FFAR3 is an SCFA receptor that can be activated by propionate (C3), butyrate (C4), and valerate (C5).
FFAR3 mediates PYY and GLP-1 secretion from endocrine and sympathetic outflow-dependent heart rate regulation and energy expenditure, while β-Hydroxybutyrate, a ketone body produced in the liver during starvation, decreases sympathetic outflow by antagonizing FFAR3.
FFAR3 is shown to couple Gαi/o pathway signaling upon activation by propionate binding in primary murine pancreatic beta cells, resulting in an inhibition of glucose-dependent insulin secretion.
FFAR3 is a 7-transmembrane (a.a.
20-40, 48-68, 89-111, 133-153, 179-199, 223-243, 259-279) GPCR, having a short N-terminal extracellular portion (a.a.
1-19) and a long C-terminal cytomplasmic tail (a.a.
280-346).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5ug/ml FFAR3/GPR41 in 10ug of human adipose tissue lysate
Immunohistochemistry (paraffin): 1:250 detected FFAR3/GPR41 in human breast cancer tissue, as well as in normal liver, pancreas, and small intestine tissue sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes an epitope in the C-terminal cytoplasmic domain of human FFAR3/GPR41.
Clone # USB：16F4.1
Isotype：IgG1,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated linear peptide corresponding to a C-terminal cytoplasmic domain sequence of human FFAR3/GPR41.
