CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors.
Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation.
GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities.
CD235a is a receptor of Hsa, a Streptococcus adhesin.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 4ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspensionOptimal dilution determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by size-exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes an epitope between amino acids 27-40 of the extracellular portion of human CD235a. Does not react with glycophorin B. Species Crossreactivity: rat
Clone # USB：JC159
Isotype：IgG1
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu Rt
Immunogen：Membrane preparation from splenic hairy cell leukemia
