CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors.
Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation.
GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities.
CD235a is a receptor of Hsa, a Streptococcus adhesin.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspensionOptimal dilution determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by size-exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes an epitope between amino acids 27-40 of the extracellular portion of human CD235a. Does not react with glycophorin B. Species Crossreactivity: rat
Clone # USB：JC159
Isotype：IgG1
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu Rt
Immunogen：Membrane preparation from splenic hairy cell leukemia
