CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors.
Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation.
GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities.
CD235a is a receptor of Hsa, a Streptococcus adhesin.
Applications:Suitable for use in Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (paraffin): 10ug/mlFlow Cytometry: 1-4ug/mlOptimal dilution determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:Recognizes an epitope between amino acids 27-40 of the extracellular portion of human CD235a. Does not react with glycophorin B. Species Crossreactivity: rat
Clone # USB:JC159
Isotype:IgG1
Calc Applications Abbrev:FC IHC
Calc Crossreactivity:Hu Rt
Immunogen:Membrane preparation from splenic hairy cell leukemia