83-5982-59　NSE (Gamma-Enolase, Neural Enolase, Neuron-specific Enolase, 2-phospho-D-glycerate Hydrolyase, ENO2) 200ul　052248

特徴

• Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias.
• These include pediatric neuroblastoma and small cell lung cancer.
• Neuron specific enolase (NSE) converts 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis.
• It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or as enolase alpha, beta and gamma.
• The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.
• NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells.
• Neurons require a great deal of energy and have high levels of glycolytic enzymes such as NSE.
• Antibodies to this NSE are useful for identifications of neuronal cell bodies, developing neuronal lineage and neuroendocrine cells.
• Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.
• Applications:Suitable for use in Immunocytochemistry, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1-10ug/mlWestern Blot: 1-5ug/mlImmunocytochemistry: 0.5-10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.