Recombinant monoclonal antibody to IL-10R with variable regions (i.e.
specificity) from the hybridoma 1B1.3a.
This clone has been widely applied in flow cytometric analyses where the detection of IL10R was needed.
Furthermore, this antibody was also successfully utilised as a blocking agent; one group used it to demonstrate that IL-10 mediates suppressive activity, by targeting its receptor IL-10R with this antibody (Wang et al., 2004).
Castro et al.
(2000) demonstrated that 1B1.3a is able to make a soluble peptide or protein antigen immunogenic for subsequent Th1 recall responses in a situation when there is an additional signal, such as LPS, activating the immune system.
Consequently, this antibody is a hugely promising agent in the potential development of vaccines for immunotherapy.
Similarly, Vicari and colleagues (2002) showed that this antibody has an enormous potential as a therapeutic anti-tumor agent when combined with the administration of CpG to stimulate the immune system.
Applications:Suitable for use in Blocking and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% Proclin 300.
Specificity：Recognizes mouse Interleukin 10 Receptor. The epitope has been localized to the ligand binding domain within the extracellular region of mouse IL-10R.
Clone # USB：1B1.3a (recomb)
Isotype：IgG1,k
Calc Applications Abbrev：FC
Calc Crossreactivity：Mo
Immunogen：Purified recombinant protein corresponding to the extracellular region of mouse Interleukin 10 Receptor.
