Ubiquitin is a small protein composed of 76aa and having a molecular weight of 8.6kD.
It exists in all eukaryotic cells and is responsible for numerous functions in the cell by post-translational modifications of substrate proteins (ubiquitination).
This process is important to numerous cellular processes including, but not limited to, apoptosis, cell cycle and division, DNA transcription, immune response, modulation of cell surface receptors, and viral infections.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:300.
Milk based blocking reagents not recommended.
Use 1% BSA in PBS or TBS polysorbate 20 as blocking buffer
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes polyubiquitinated conjugates, but does not react with free ubiquitin or monoubiquitinated conjugates. Species independent.
Clone # USB：FK1
Isotype：IgM
Calc Applications Abbrev：WB
Immunogen：Crude preparation of polyubiquitinated-lysozyme
