Heat shock protein 70s (Hsp70s) are abundant and stress-inducible 70kD molecular chaperone proteins encoded by a highly conserved, multigene family.
They are monomeric proteins that can be divided into two functional domains: an N-terminal ATPase domain and a substrate binding domain that contains a highly conserved EEVD motif at its C-terminus.
Hsp70s are found in the cytosol, nuclei, endoplasmic reticulum, mitochondria, and chloroplasts of eukaryotes, as well as in bacteria.
They function as molecular chaperones that assist in a wide range of cellular processes, including refolding of aggregated or misfolded proteins, co- and post-translational folding and assembly of nascent peptides, membrane translocation of secretory and organellar proteins, controlling activity of regulatory nuclear receptors, kinases and transcription factors, as well as cooperativity with the Hsp90 chaperone system in eukaryotes.
The Hsp70 chaperone cycle is ATP-dependent and initiated by transient interaction of the Hsp70 substrate binding domain with hydrophobic regions within a peptide or protein.
It consists of an alteration between the low-affinity ATP-bound state with fast rates of substrate exchange and the high-affinity ADP bound state with slow rates of substrate exchange.
Hsp70s are subject to a variety of post-translational modifications and their expression is upregulated under conditions of cellular stress and in a variety of disease states.
Applications:Suitable for use in Immunoprecipitation, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000Immunohistochemistry (FFPE): 1:40 using heat induced antigen retrieval in citrate buffer, pH 6.0
Western Blot: 1:200Immunoprecipitation: 5ug/testOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HSPA1B. Species Crossreactivity: mouse. Does not react with GRP78 or rat Hsp70.
Clone # USB：7F6
Isotype：IgG1
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Full length recombinant protein corresponding to human Hsp70
