Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human.
MBP is a homodimeric protein that is found in both the central and the peripheral nervous system.
It is one of the most abundant protein component of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane.
It is responsible for adhesion of the cytosolic surfaces of multilayered compact myelin.
MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids.
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
MBP expression begins abruptly in 14-16-week-old fetuses.
Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult.
MBP citrullination by peptidylarginine deiminase (PAD) leads to incomplete protein-lipid bilayer interactions and increases its vulnerability to proteolytic enzymes, resulting in disorganization of the myelin sheath in the central nervous system.
Citrullinated MBP (citMBP) has been suggested to be a hallmark of demyelination.
MBP citrullination has frequently been demonstrated in various CNS disorders.
citMBP at R25, R122, and R130 is reported in the brains of subjects with Creutzfeldt-Jakob disease (CJD).
Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1ug/ml detected MBP (Citrulline R122) in GST-tagged myelin basic protein (MBP) treated with human Peptidyl Arginine Deiminase 2 (PAD-2) (200ng for 4 h at 37℃)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human and mouse myelin basic protein citrullinated on residue 122. Targets an epitope with in 19 amino acids from the C-terminal half.
Clone # USB：1H1
Isotype：IgG1,k
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated linear peptide corresponding to 19 amino acids from isoform 5 of human Myelin Basic Protein with citrullination on residue 122.
