83-5982-41　Growth Associated Protein 43 (GAP43) 100ul　052233

特徴

• GAP43 is an abundant protein which is found heavily concentrated in developing neurons, in particular at the growing tips, the growth cones.
• One group discovered it since it becomes unregulated during the regeneration of the toad optic nerve, and named it “growth associated protein 43”, the 43 referring to the apparent molecular weight on SDS-PAGE gels (1).
• GAP43 is very highly charged and does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, since human GAP43 is 238 amino acids giving a real molecular weight 24.8kDa.
• The same GAP43 preparation will also give a different SDS-PAGE molecular weight depending on the percentage acrylamide content of the gel, the protein appearing relatively larger on gels with higher acrylamide concentration.
• GAP43 proteins from different species also may run at different apparent molecular weights on the same gel.
• Partly due to these unusual features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57, the numbers 46, 50 and 57 reflecting the apparent SDS-PAGE molecular weight (2).
• GAP43 is a major protein kinase C substrate and binds calmodulin avidly, this being mediated by an N-terminal IQ calmodulin binding motif (3).
• GAP43 may be anchored to the plasma membrane by reversible palmitoylation on two Cys residues close to the N-terminus (4).
• Knock out of the GAP43 gene in mice is lethal early in postnatal life and is associated with defects in axonal pathfinding (5).
• GAP43 is one of a large family of “intrinsically disordered proteins” which typically have little defined structure unless they are bound to a more structured partner (6).
• Applications:Suitable for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence (IC): 1:1000-1:5000Western Blot: 1:5000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.