83-5982-33　AIDA1 (Ankyrin Repeat and Sterile alpha Motif Domain-containing Protein 1B, Amyloid-beta Protein Intracellular Domain-associated Protein 1, EB-1) 100ug　052227

特徴

• Ankyrin repeat and sterile alpha motif domain-containing protein 1B (UniProt: P0C6S7; also known as Amyloid-beta protein intracellular domain-associated protein 1, AIDA-1, E2A-PBX1-associated protein, EB-1) is encoded by the Anks1b gene (Gene ID: 314721) in rat.
• AIDA-1 is an adaptor protein that has been implicated in Cajal body and nucleolar function.
• It is expressed throughout the brain and is highly expressed in the hippocampus.
• It is one of the most abundant proteins at postsynaptic densities where it is reported to bind to PSD95 and associates with NMDA receptors (NMDARs).
• In the brain, it interacts with amyloid b precursor protein and plays a role in brain development.
• AIDA-1 preferentially associates with GluN2B-containing NMDARs and plays a role in their transport from the endoplasmic reticulum (ER).
• Loss of AIDA-1 results in the accumulation of GluN2B in the ER and decreases GluN2B and increases GluN2A distribution at the synapse.
• However, loss of AIDA-1 does not affect AMPAR-mediated synaptic transmission or the probability of glutamate release.
• AIDA-1 has seven Ankyrin repeats in its N-terminal half and has two closely juxtaposed sterile alpha motif (SAM) domains (aa 810-876 and 884-949).
• Within the second SAM domain resides the nuclear localization signal (aa 935-938).
• Mutations in Anks1b gene are known to affect GluN2B function and are linked to the pathogenesis of neuropsychiatric disorders.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 detected AIDA1 in rat testis tissue lysates under non reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃.
• For long-term storage, aliquot and store at 4℃.
• Do not freeze.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Further dilutions can be made in assay buffer.