83-5982-26　CD261 (TRAIL Receptor 1, TRAIL-R1, Death Receptor 4, DR4, APO2, TNFRSF10A) (Low Endotoxin/Azide Free) 100ug　052221X

特徴

• TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1.
• The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family.
• DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues.
• Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms.
• Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).
• Applications:Suitable for use in Flow Cytometry, Functional Assays, Immunocytochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Functional Application: 052221X blocks apoptosis triggered by a ligand (TRAIL).
• Plastic-immobilized (cross-linked) antibody induces apoptosis in sensitive cells.
• Recommended dilution of antibody: 2-3ug/ml in cultivation medium.
• The final concentration of TRAIL is 20-200ng/ml.
• Add the antibody 15 min before addition of TRAIL
• Flow Cytometry: 2-4ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.