Reticulon-4 (UniProt: Q99P72; also known as Neurite outgrowth inhibitor, Nogo protein) is encoded by the Rtn4 (also known as Kiaa0886, Nogo) gene (Gene ID: 68585) in murine species.
NOGO proteins are required for the formation and stabilization of endoplasmic reticulum (ER) tubules.
They are also involved in nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins.
Three isoforms of NOGO have been described that share a common C-terminal domain but differ in their N-terminal sequence.
NOGO-A is the longest isoform and is considered as a developmental neurite growth factor.
It acts as a negative regulator of axon-axon adhesion and growth, but also acts as a facilitator of neurite branching.
In the adult central nervous system (CNS), it is involved in down-regulation of growth, stabilization of wiring, and restriction of plasticity.
It can also serve as a negative regulator of CNS angiogenesis by blocking spreading, migration, and sprouting of primary brain microvascular endothelial cells.
Isoform B mainly functions in the endothelial cells and vascular smooth muscle cells.
It promotes the migration of endothelial cells but inhibits the migration of vascular smooth muscle cells.
Isoform C regulates cardiomyocyte apoptosis under hypoxic conditions.
This clone has been use to show that knockdown of NOGO-A reduces the number of axonal collateral branches in olfactory bulb.
Applications:Suitable for use in Immunohistochemistry.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：hamster
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes mouse Reticulon-4. Does not react with other isoforms. It is shown to strongly bind olfactory bulb axons in the lateral olfactory tract as well as some other growing axons in nervous system.
Clone # USB：NG1
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Mo
Immunogen：Lateral olfactory tract of E14.5 mouse embryos fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS), washed and suspended in PBS.
