C-C motif chemokine 22 (UniProt: O00626; also known as CC chemokine STCP-1, MDC(1-69), Macrophage-derived chemokine, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1, CCL22) is encoded by the CCL22 (also known as MDC, SCYA22, A-152E5.1) gene (Gene ID: 6367) in human.
MDC/CCL22 is a member of the intercrine beta family that plays a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology.
It is highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus.
It is reported to be chemotactic for monocytes, dendritic cells, and natural killer cells.
It displays mild chemoattractant activity for primary activated T-lymphocytes but is a potent chemoattractant for chronically activated T-lymphocytes.
Its signaling activity is achieved through the C-C chemokine receptor type 4 (CCR4).
It acts as a mediator of lung inflammation after hemorrhage and resuscitation.
MDC/CCL22 is synthesized with a signal peptide (aa1-24) that is subsequently cleaved off.
It can be proteolytically cleaved into three processed forms that are known as MDC (3-69); MDC (5-69); and MDC (7-69) (numbered without the signal peptide).
Applications: Suitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (paraffin): 1:50 detected CCL22/MDC in human skin and human small intestine tissue sections using heat-induced epitope retrieval (HIER)
Western Blot: 1:1000 dilution detected human recombinant MDC-CCL22 protein
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% sodium azide
Specificity：Recognizes human CC-motif chemokine 22 (CCL22).
Clone # USB：6F5.7
Isotype：IgG2a,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein fragment of human CCL22 corresponding to 67aa in the mature protein.
