The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers.
The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.
It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways.
Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading.
Several transcript variants encoding different isoforms have been found for this gene.
Applications:Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1:200-1:400 detected LIMS1 in HeLa cells
Immunofluorescence: 1:200-1:1000 detected LIMS1 in HepG2 cells
Western Blot: 1:500-1:2000 detected LIMS1 in A549, Jurkat and HeLa cell lysates
ELISA: 1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Ascites
Purity：Ascites
Form：Supplied as a liquid, 0.03% sodium azide
Specificity：Recognizes human PINCH.
Clone # USB：5G7
Isotype：IgG1
Calc Applications Abbrev：E FC IC IF WB
Calc Crossreactivity：Hu
Immunogen：Purified recombinant protein fragment corresponding to human PINCH, expressed in E. coli.
