Pinch-1 (Particularly interesting new Cys-His protein 1) is also known as LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1).
Pinch-1 is expressed in most tissues except for brain.
Pinch-1 is an adhesion protein and is part of the ILK-PINCH complex that is involved in integrin and growth factor signaling by coupling surface receptors to downstream signaling molecules related to the regulation of cell survival, cell proliferation and cell differentiation.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5ug/ml detected Pinch-1 in 10ug of human heart tissue lysate
Immunohistochemistry (paraffin): 1:250 detected Pinch-1 in human renal tubules and mouse renal tubules tissue.
Requires HIER using sodium citrate buffer pH6.0 prior to staining
Optimal dilutions to be determined by the researcher.
Control:Human heart tissue lysate
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.05% sodium azide
Specificity：Recognizes human Pinch-1. Species Crossreactivity: mouse
Clone # USB：9D2.1
Isotype：IgM,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated linear peptide corresponding to human Pinch-1.
