SH2 domain-containing protein 1A (UniProt O60880; also known as Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP) is encoded by the Sh2d1a (also known as Xlp) gene (Gene ID 20400) in murine species.
SAP/SH2D1A is a 628-amino acid (isoform A) adaptor protein expressed in T cells, natural killer (NK) cells, NK-T cells, and some B cells.
SAP/SH2D1A is made up almost exclusively of a Src homology 2 (SH2) domain (aa6-104) and plays an important role in multiple immune cell functions, including T helper 2 (TH2) cytokine production, T cell-dependent antibody production, follicular T helper (TFH) cell differentiation, NK-T cell development, CD8+ T cell- and NK cell-mediated cytotoxicity.
SAP couples signaling lymphocytic activation molecule (SLAM) family receptors to tyrosine phosphorylation signals through its interaction with the SH3 domain of Src family protein tyrosine kinase Fyn.
In addition, SAP can prevent SH2 domain-containing negative regulators, such as phosphatases SHIP-1 and SHP-1, from inactivating SLAM-related receptors by blocking their interaction with the receptors.
Evidence also shows that SAP/SH2D1A regulates T cell-dependent antibody production and TFH cell differentiation independent of Fyn.
SAP/SH2D1A gene is mutated and inactivated in X-linked lymphoproliferative disease, a rare human immunodeficiency characterized by an uncontrolled immune response to Epstein-Barr virus infection.
Applications:Suitable for use in Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 0.1ug detected SAP/SH2D1A in mouse splenocytes
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes mouse SH2D1A. Does not react with EAT-2 or ERT (Roncagalli, R., et al. (2005). Nat. Immunol. 6(10):1002-1010). 052197 immunostained wild-type, but not sh2d1a-knockout murine peripheral blood CD3+CD4+ lymphocytes (Zhong, M.C., and Veillette, A. (2013). Mol. Cell. Biol. 33(6):1223-1232). Species Crossreactivity: human.
Clone # USB：1A9
Isotype：IgG2a,k
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Hu Mo
Immunogen：GST-tagged recombinant protein corresponding to full-length mouse SAP.
