Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55kD glycoprotein secreted by the liver and fat tissue.
It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1).
Mature human ANGPTL4 shares 26-30% amino acid (aa) sequence identity with ANGPTL1, 2, 3, 5, 6, and 7.
It shares approximately 75% aa sequence identity with mouse and rat ANGPTL4.
The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2).
This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4).
In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4).
In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4).
Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5).
Its expression in adipose tissue is induced by fasting and suppressed by feeding (6).
In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8).
The N-terminal fragment can function as an angiogenesis inhibitor (7, 8).
In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10).
ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12).
The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 0.25ug/ml/10e6 cells using CHO cells transfected with hANGPTL4 and mCherry vs irrelevant cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human Angiopoietin‑like Protein 4.
Clone # USB：24J4
Isotype：IgG2b
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly26-Ser406 from human ANGPTL4, expressed in NS0 cells.
