Acetylcholine receptor subunit alpha (UniProt: P02708; AChR nicotinic alpha 1) is encoded by the CHRNA1 (also known as ACHRA, CHNRA) gene (Gene ID: 1134) in human.
Nicotinic Acetylcholine Receptor is a member of the ligan-gated ion channel family.
It is a multi-pass membrane protein on the post-synaptic cell membrane.
Nicotinic Ach receptor is a pentamer of two alpha chains and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.
Two isoforms of this receptor have been described that are produced by alternative splicing.
Isoform 1 is only expressed in skeletal muscle and isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.
It contains an extracellular domain (aa21-255), four helical domains (aa 256-280; 288-306; 322-341; and 454-472) and a cytoplasmic domain (aa342-453).
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Defects in CHRNA1 gene are linked to Myasthenic syndrome, which is characterized by muscle weakness affecting the axial and limb muscles, and the ocular muscles that lead to ptosis and opthalmoplegia.
The alpha subunit is the main focus for antibody binding in myasthenia gravis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.5ug/ml detected Nicotinic Acetylchone Receptor alpha 1 in 10ug of SW480 cell lysate
Immunohistochemistry (paraffin): 1:50 detected Nicotinic Acetylchone Receptor alpha 1 in human colon cancer tissue sections using heat-induced epitope retrieval (HIER)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human nicotinic acetylcholine receptor alpha. Species Crossreactivity: rat. Targets an epitope within 97aa from the C-terminal half.
Clone # USB：7C8.2
Isotype：IgG2a,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：GST/His-tagged recombinant protein fragment corresponding to 97aa from the C-terminal half of human nicotinic acetylcholine receptor alpha.
