83-5981-99　Glutamate Decarboxylase 67 (GAD67) (Biotin) 100ul　052192

特徴

• Gutamic acid decarboxylase (GAD; E.C.
• 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets.
• Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary.
• The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999).
• GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production.
• GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production.
• GAD expression changes during neural development in rat spinal cord.
• GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999).
• In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995).
• GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000).
• Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
• Applications:Suitable for use in Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunocytochemistry: 1:100 detected GAD67 in rat E18 primary cortex cells
• Immunohistochemistry (Paraffin): 1:25 detected GAD67 in rat cortex tissue
• Optimal dilutions to be determined by the researcher.
• Control:Rat cortex tissue
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.