Interferon regulatory factor 3 (IRF3) is a 50 kDa, 427aa, member of the IRF family of proteins.
Human IRF3 is a transcription factor that binds the interferon-sensitive response element (ISRE) and is activated by Toll-like receptors, TLR3 and TLR4.
Viral entry or engagement of TLR3 or TLR4 stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production
Applications:Suitable for use in Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml in lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell lineFlow Cytometry: 2.5ug labels 10e6 cells in Daudi Human Cell LineOptimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer,
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human IRF3.
Clone # USB：24J3
Isotype：IgG2b
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa206-427 from human IRF3, expressed in E. coli.
