Natural cytotoxicity triggering receptor 1 (UniProt: O76036; also known as Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, hNKp46, CD335) is encoded by the NCR1 (also known as LY94) gene (Gene ID: 9437) in human.
NK-p46 is a single-pass type I membrane protein that belongs to the natural cytotoxicity receptor (NCR) family and serves as a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis and elimination of virus-infected cells.
It is considered as a primary activating receptor mediating NK cell antiviral function.
It is involved in the functional recognition of viral HA and hemagglutinin-neuraminidases.
NKp46 is shown to be predominantly expressed in the nasal lavage of patients infected with respiratory viruses, but not in normal healthy subjects.
Following the initiation of an NK-target cell interaction, NKp46 is reported to cluster at the cell membrane, specifically at the immune synapse where it mediates cytoskeletal rearrangement and cellular polarization.
Human NK-p46 is synthesized with a signal peptide of 21 amino acids, which is subsequently cleaved off.
The mature form has an extracellular domain (aa 22-258), a helical domain (aa 259-279), and a cytoplasmic domain (aa 280-304).
It also has two Ig-like domains (aa 34-118 and 129-211) termed as D1 and D2.
Five different isoforms of NKp46 have been described that are produced by alternative splicing.
Applications:Suitable for use in Flow Cytometry and ELISA.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 4ug/ml detected NKp46 (CD335) in NK92 cells transfected with human NKp46 (Courtesy of Angel Porgador, Ph.D., Faculty of Health Sciences, Ben Gurion university of the Negev, Israel)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Specificity：Recognizes whole human NKp46 and NKp46-D1. Does not react with NKp46-D2. It targets an epitope with in the first 100 amino acids from the extracellular domain.
Clone # USB：461-G1
Isotype：IgG1,k
Calc Applications Abbrev：E FC
Calc Crossreactivity：Hu
Immunogen：Human IgG1 Fc-fused to a recombinant fragment corresponding to the first 100 amino acids from the extracellular domain of human NKp46.
