IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes.
The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons.
The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha.
It will compete with either factor for receptor binding, but does not interact with either one.
Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines.
The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form.
The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo.
Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
Applications:Suitable for use in ELISA and Neutralization.
Other applications not tested.
Recommended Dilutions:ELISA (capture): 2-8ug/ml with 052180 as detection antibodyNeutralization: Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha /IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line.
The ND50 is typically 10-20ug/mL in the presence of 50ng/mL recombinant human IL‑1ra/IL‑1F3, 50pg/mL recombinant human IL‑1 alpha /IL‑1F1
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from ascites. Endotoxin: ≤0.1EU/ug (LAL).
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 2mL sterile PBS.
Specificity：Recognizes human IL‑1ra/IL-1F3. <0.2% cross-reactivity with recombinant mouse IL‑1ra. Does not react with hIL‑1 alpha, rpIL‑1 alpha, rmIL‑1 alpha, rrIL‑1 alpha, rhIL‑1 beta, rpIL‑1 beta, rmIL‑1 beta, rrIL‑1 beta, rhIL‑1 RI, or rhIL‑1 RII.
Clone # USB：24J1
Isotype：IgG2a
Calc Applications Abbrev：E N
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg26-Glu177 from human IL‑1ra/IL-1F3, expressed in E. coli
