Coagulation factor VIII (UniProt: P00451; also known as Antihemophilic factor, AHF, Procoagulant component) is encoded by the F8 (also known as F8C) gene (Gene ID: 2157) in human.
Factor VIII is a heavily glycosylated plasma protein that is synthesized with a signal peptide (aa1-19) and is further cleaved and processed to generate Factor VIIIa heavy chain of 200kD (aa20-1332), Factor VIIIa heavy chain of 92kD (aa20-759), Factor VIII B chain (aa760-1332), and the Factor VIIIa light chain (aa1668-2351).
In plasma, Factor VIII is thought to be associated in a complex with the highest molecular weight multimers of Von Willebrand protein in an inactive form and following injury or wound it dissociates from Willebrand factor and is activated (Factor VIIIa).
Along with calcium and phospholipid, it acts as a cofactor for Factor IXa, which is responsible for converting Factor X to Xa.
Its concentration in plasma ranges from 100-200ng/ml.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: IndirectOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity：Recognizes the light chain of human Factor VIII (83kD). Does not cross-react with von Willebrand factor.
