5-hydroxytryptamine receptor 2A (UniProt P28223; also known as 5-HT-2, 5-HT-2A, 5-HT2A, Serotonin receptor 2A) is encoded by the HTR2A (also known as HTR2) gene (Gene ID 3356) in human.
Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals.
In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels.
Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite.
A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT.
5-HT2A receptor, which couples to Gq/11 to increase intracellular calcium, is widely expressed at central and peripheral sites of 5-HT action and contributes to many of the physiological effects of 5-HT.
The hallucinogenic activity of LSD is mediated in part by its action as a partial to full agonist of 5-HT2A receptor, and the activity of atypical antipsychotics such as clozapine appears to be mediated in part by antagonism against 5-HT2A receptor.
Human 5-HT2A receptor contains four extracellular domains (a.a.
76-99, 111-132, 149-171, 192-215, 234-254, 325-346, 363-384), and four cytoplasmic domains (a.a.
100-110, 172-191, 255-324, 385-471).
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (FFPE): 1:50 detected 2A/HTR2A in human cerebral cortex, mouse cerebral cortex, and rat testis tissue sections.
Requires HIER
Western Blot: 0.5ug/ml detected 2A/HTR2A in 10ug of human cerebral cortex tissue lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes Serotonin Receptor 2A. Species Crossreactivity: mouse and rat
Clone # USB：9B11.1
Isotype：IgG2a,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：GST-tagged recombinant protein fragment corresponding to the third cytoplasmic domain of human Serotonin receptor 2A/HTR2A.
