5-hydroxytryptamine receptor 1B (UniProt P28222; also known as 5-HT-1B, 5-HT-1D-beta, 5-HT1B, S12, Serotonin 1D beta receptor, Serotonin receptor 1B) is encoded by the HTR1B (also known as HTR1DB) gene (Gene ID 3351) in human.
The 5-HT receptors are grouped according to their antagonist susceptibilities and their affinities for 5-HT.
The 5-HT1 receptors are a subfamily of 5-HT receptors that were originally classified according to their inhibition of adenylyl cyclase, degree of sequence similarity and their overlapping pharmacological specificities.
The subfamily has five different receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT1F that can couple to Gi/Go and mediate inhibitory neurotransmission.
5HT-1B receptor belong to the family of G-protein coupled receptors that are distributed in brain cortex, striatum, amygdala, medulla, hippocampus, caudate nucleus and putamen.
5-HT-1B also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide.
Ligand binding causes a conformation change in the receptor that triggers signaling via G-proteins, which modulates the activity of down-stream effectors, such as adenylate cyclase.
Signaling via 5-HT-1B inhibits adenylate cyclase activity.
5-HT1B receptor is known to be involved in thermoregulation, respiration, appetite control, sexual behavior, and aggression.
5-HT-1B receptor are therapeutic targets for antimigraine drugs and for treatment of bipolar disorders, autism, and anti-aggressive agents.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (FFPE): 1:50-1:250 detected serotonin receptor 1B in human (cerebellum and cerebral cortex) and rat (cerebral cortex and medulla) brain tissue sections.
Requires HIER
Western Blot: 1ug/ml detected serotonin receptor 1B in 10ug of mouse brain tissue lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes an epitope within the third cytoplasmic domain of human, mouse, and rat serotonin receptor 1B.
Clone # USB：1C2.1
Isotype：IgG1,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：GST-tagged recombinant protein fragment corresponding to the thrid cytoplasmic domain of human serotonin receptor 1B.
