Chlamydia trachomatis is a Gram-negative bacterium that is responsible for sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV).
Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants.
These are identified based on serological reactivity of the epitopes on their outer membrane.
Intracellularly chlamydia replicates within a vacuole.
Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles.
This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell.
Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate.
052166 specifically detects major outer membrane protein (MOMP) from Chlamydia genus.
It displays strong reactivity with Chlamydial serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
MOMP, a cysteine-rich molecule, plays a vital role in maintaining the structural and functional properties of the chlamydial outer membrane.
It contains determinants that confer type, subspecies, and species antigenic properties to the protein.
Applications:Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Dot Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml detected chlamydial Chlamydial MOMP in lysates from HeLa cells infected with Chlamydia trachomatis serovar L2 (LGV 434 L2)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes the outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
