Adeno-associated viruses (AAV) are small, non-enveloped, non-pathogenic viruses that package a linear single-stranded DNA genome.
AAV belong to the family Parvoviridae and productive infection by AAV occurs only in the presence of a helper virus, which could either be adenovirus or herpesvirus.
AAV are recognized by glycosylated cell surface receptors of the host cell.
This triggers internalization of the virus via clathrin-mediated endocytosis and then AAV traffic through the cytosol mediated by the cytoskeletal network.
After successful infection, two stages of AAV life cycle have been described - a lytic stage and a lysogenic stage.
In the presence of helper virus, the lytic stage ensues and during this period, AAV undergoes productive infection with genome replication, viral gene expression, and virion production.
The lysogenic stage is established in host cells in the absence of a helper virus and AAV integrates into the host genome at specific site that contains a Rep binding element.
AAV serotype 5 (AAV5) is one of the most divergent of the AAVs and it utilizes 2,3-linked sialic acid-containing glycans as primary host cell receptors.
The virus capsids are assembled from 60 subunits of three overlapping capsid viral proteins: VP1; VP2; and VP3 in a 1:1:10 ratio.
The individual VPs are expressed within the same open reading frame and share a C terminus.
VP1 and VP2 represent N-terminal extended forms of VP3.
052141 is reported to bind to the 3-fold capsid region and exhibits high specificity of AAV5.
Arginine 483 is reported to be the most critical contact residue for its binding (ref.
1, ref.
2).
Applications:Suitable for use in ELISA, Dot Blot and Neutralization.
Other applications not tested.
Recommended Dilution:Dot Blot: 10ng detected Adenovirus 5, but not AV8 and AAV9 virus like particlesOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes Adeno-associated virus 5 binds to the 3-fold protrusions in the capsid protein.
Clone # USB：HL2476
Isotype：IgG2b,k
Calc Applications Abbrev：DB E N
Immunogen：Recombinant protein corresponding to Adeno-associated virus 5 (AAV5) virus-like particles.
