83-5981-63　RNA Polymerase II Subunit B1, CTD phosphorylated (Ser7) 100ug　052136

特徴

• RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex.
• As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates.
• The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript.
• This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats.
• Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.
• In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated.
• Ser-7 is phosphorylated before initiation of transcription at promoter regions.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.25ug/ml detected RNA polymerase II CTD on 10ug of γ-PPase untreated and treated NIH/3T3 cell lysates
• Optimal dilutions to be determined by the researcher.
• Control:γ-protein phosphatase (γ-Ppase) untreated and treated NIH/3T3 cell lysates.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.