Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members.
It is an integral membrane protein with 12 transmembrane domains.
Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells.
It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state.
Applications:Suitable for use in Flow Cytometry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: 8-25ug/ml using immersion fixed beta TC-6 mouse beta cell insulinoma cell lineFlow Cytometry: 2.5ug labels 10e6 cells using beta TC-6 mouse beta cell insulinoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 400ul sterile PBS.
Specificity：Recognizes mouse Glut2-transfected NS0 cells but not NS0 control transfectants. Also detects Glut2 on mouse insulinoma beta TC-6 cells.
Clone # USB：24I7
Isotype：IgG2b
Calc Applications Abbrev：FC IC
Calc Crossreactivity：Mo
Immunogen：CHO cell line transfected with mouse Glut2. Epitope: Met1-Val523
