These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11.
The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules.
This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits.
CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum.
It is never expressed on B or NK cells.
Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22kD and a weak 28kD zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7).
Applications:Suitable for use in Immunoprecipitation and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 2-5ug/ml using peripheral blood lymphocytes and jurkat human leukemia T cell line as positive controlsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by size-exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Biotin.
Specificity：Recognizes an extracellular epitope on gamma-epsilon and delta-epsilon dimers of human CD3 complex
