CD74 (the MHC II-associated invariant chain, Ii) is a type II transmembrane protein expressed in antigen-presenting cells, that serves as MHC II chaperone which promotes MHC II trafficking from the ER to endocytic compartments, prevents peptide binding in the ER and contributes to peptide editing in the MHC II compartment; it is also an accessory signaling molecule implicated e.g.
in malignant B cell proliferation.
Stimulation of cell surface CD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the S phase.
CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44.
Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.
Applications:Suitable for use in Flow Cytometry.
Other applications have not been tested
Recommended Dilution:Flow Cytometry: 20ul labels 10e6 cells in 100ul solution or whole bloodOptimal dilution determined by the researcher.
Excitation Laser:Blue (488nm)
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by size-exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).
