MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 0.25ug detected MHC class II (I-A/I-E), FITC in 10e6 C57Bl/6 splenocytes
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Highly Purified
Purity：Purified by size-exclusion chromatography from tissue culture supernatant
Form：Supplied as a liquid in 10mM monosodium phosphate, 150mM sodium chloride, 0.1% gelatin, pH 7.2, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. Does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7. 052095 may be used for analysis of mouse cells expressing MHC Class II alloantigens as described
