The stat proteins (Stat1-6) function both as cytoplasmic signal transducers and as activators of transcription in response to cytokines and growth factor receptors.
Stat3 is expressed as two variants, Stat3α (86kD) and Stat3b (79kD) that can differ in expression and activity depending on cell type, activation pathway, and cell maturation stage.
Both are activated by phosphorylation at Tyr-705, which induces dimerization, nuclear translocation and DNA binding.
Stat3α (86kD) transcriptional activation may be regulated by phosphorylation at Ser-727 through the MAPK pathway, while Stat3β lacks this serine site
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity：Recognizes human Stat3 at N-terminal. Detects 86 and 79 kD bands corresponding to Stat3 isoforms on SDS-PAGE immunoblots of human A431 cells.
Clone # USB：M263
Isotype：IgG1
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to amino acid residues in the N-terminal region of human Stat3. This sequence has high homology to the conserved regions in rat and mouse Stat3 α and β.
